منابع مشابه
Quantitative analysis of phospholipids by 31P-NMR.
High-field 31P nuclear magnetic resonance spectroscopy was used to quantitate phospholipids in mixtures in organic solvents. The sample is dissolved in chloroform-methanol and analyzed at 161.7 MHz with decoupling of the protons. Signals were identified using authentic compounds, and their relative distribution was measured in mole percent. The method has good accuracy and reproducibility, and ...
متن کامل31P NMR of phospholipid glycerol phosphodiester residues.
Saponification of extracted tissue phospholipids yields a set of isolated glycerol 3-phosphoryl phospholipid polar headgroups from which semi-quantitative 31P NMR spectra can be obtained. The resonance signals from these molecules, which frequently have been reported as uncharacterized phosphate signals observed in perchloric acid extracts of tissue, can be used as an aid in the characterizatio...
متن کامل31P NMR of phosphate and phosphonate complexes of metalloalkaline phosphatases.
31P NMR spectra of phosphate and phosphonate complexes of Escherichia coli alkaline phosphatase have been obtained by Fourier transform NMR methods. One equivalent of P1i, bound to Zn(II) alkaline phosphatase, pH 8, gives rise to a single 31P resonance 2 ppm downfield from that for Pi, and assignable to the noncovalent complex, E-P. Inorganic phosphate in excess of 1 eq per enzyme dimer gives r...
متن کامل31P NMR studies of spinach leaves and their chloroplasts.
An experimental arrangement is described which enables high quality 31P NMR spectra of compressed spinach leaf pieces to be continuously recorded in which all the resonances observed (cytoplasmic and vacuolar Pi, glycerate-3-P, nucleotides) were sharp and well resolved. 31P NMR spectra obtained from intact chloroplasts showed a distinct peak of stromal Pi. An upfield shift of the stromal Pi res...
متن کاملApplication of 31P NMR to model and biological membrane systems.
Proton, deuterium and carbon magnetic resonance techniques have been widely used to study the hydrocarbon chain region of model bilayer systems and biological membranes (for a review see :[l]). More recently, phosphorus magnetic resonance (31P NMR) has been introduced to study the polar headgroup region of membranes [2-7]. In this communication we show how 31P NMR can define an order parameter ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Proceedings of the Japan Academy, Series B
سال: 1980
ISSN: 0386-2208,1349-2896
DOI: 10.2183/pjab.56.597